We also conducted a genome-wide analysis of DNA methylated sites in RPMI7951, C32, Malme-3M, and SK-MEL-28 melanoma lines and found that the IRAK-M promoter region were hypomethylated in RPMI7951 but hypermethylated in C32, Malme-3M, and SK-MEL-28 cells (Supplementary Fig. 4 and Supplementary Table 2). This evidence concerns the gene IRAK3 and melanoma.