As shown in Fig. 3h, ARS2 knockdown in X01 cells significantly decreased relative luciferase expression, indicating reduced transcriptional activity of ARS2 toward MGLL. Collectively, these data demonstrate that a number of genes are potentially regulated at the transcriptional level by ARS2, and specifically identify MGLL as a novel target of ARS2 in GSCs, consistent with the role of MAGL in aggressive cancers. The gene discussed is MGLL; the disease is cancer.