While our in vitro and in vivo data strongly supported the ability of ARS2 to concurrently drive glioma stemness and polarization into M2 macrophages, the complete elucidation of the details of ARS2 driven GSC-macrophages cross-talk will recessively require the use of highly specialized macrophages depleted immunodeficient mice. The gene discussed is SRRT; the disease is central nervous system cancer.