Furthermore, the administration of 3,5-T2 to HFD rats seems to protect animals from the onset of non-alcoholic fatty liver disease (NAFLD) [191], although this finding has been challenged by results obtained in Sprague–Dawley rats fed an unsaturated fat diet, in which 3,5-T2 failed to improve NAFLD and insulin sensitivity [200]. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.