EPHA2 and osteosarcoma: After 24 and 48 h of transfection, we demonstrated that down-modulation of EphA2 significantly reduced cell migration in all transfected osteosarcoma cell lines, compared to scrambled controls (p < 0.05 in HOS and MNNG-HOS, p < 0.01 in U-2 OS and p < 0.001 in KHOS/NP, Figure 4D,E).