Even though the A375 melanoma cells were grown in full media, the PIKfyve inhibitor, as well as the pyridinyl imidazole compounds SB202190 and SB590885, all seemed to disrupt lysosomal mTOR targeting as the mTOR staining pattern changed from dot-like structures to diffuse cytoplasmic staining (Figure 5A). The gene discussed is PIKFYVE; the disease is melanoma.