In our experiments, the PIKfyve inhibitor YM201636 and pyridinyl imidazole inhibitors SB202190 and SB590885 all promoted nuclear localization of TFEB (Figure 5B), indicating that the drug-induced disruption of mTOR lysosomal tethering could inhibit mTORC1 activity in melanoma cells. The gene discussed is PIKFYVE; the disease is melanoma.