LGALS3 and anaplastic astrocytoma: In total, out of all 304 glioma cases, LGALS3 protein expression was positive in 125 glioma cases (41.1%, 125/304), with 69.2% (9/13) in WHO I (pilocytic astrocytoma), 9.8% (8/82) in WHO II (diffuse astrocytoma and oligodendroglioma), 34.2% (26/76) in WHO III (anaplastic astrocytoma and oligodendroglioma), and 61.7% (82/133) in WHO IV (glioblastoma) (Figure S2A).