LMX1B and nail-patella syndrome: In spite of the implicated role of lmx1b genes in pathogenic features of the Nail-Patella Syndrome (McMahon et al., 2009; Liu and Johnson, 2010), both lmx1b.1 and lmx1b.2 genes did not display classical AS expression patterns in our WISH assay at early timepoints, 12–48 hpf, but are detected in the AS at later stages, 72 hpf+ (Figures 1E,F).