Interestingly, relative activation of GZMB showed strong inverse correlations to NC packing, including around CD8+ cells (Figure 6), suggesting that spatial distribution of activated CD8+ cells relative to other CD8+ cells and negative cells may affect lethality of cytotoxic T cells, specifically that suppressive effects of exhausted CD8+ cells may decrease the cytotoxicity of activated CD8+ T cells (CTL), despite these CTLs being in close proximity to negative cells that undoubtedly include tumor cells. The gene discussed is CD8A; the disease is neoplasm.