CD9 and infection: This communication evidenced the isolation and enrichment of EVs inTheileria infections in buffaloes, confirming the literaturedata about EVs in parasitic infections [57].Isolation of vesicles was conducted by means of size exclusion chromatography andidentification of EVs was assessed through western blotting by the presence oftetraspanin CD9 and membrane lipids raft-associated Flotllin-1 [4].