The qRT-PCR analysis of 35 key genes including 21 hub genes and 14 other important upregulated genes in our own AKI samples (Figure 8A) showed that 31 genes were validated as significantly upregulated, 3 genes were significantly downregulated, and Krt20 were identified as the top 1 upregulated genes with a log2 (fold change) larger than 10 in all these 35 genes. This evidence concerns the gene KRT20 and acute kidney injury.