To confirm whether OMT-targeted inhibition of microglia activation was mediated through the HMGB1/TLR4/NF-κB signaling pathway, firstly, the expressional levels of HMGB1, TLR4, and CathD were evaluated by Western blot analysis in midbrains of MPTP-induced PD mice. This evidence concerns the gene HMGB1 and Parkinson disease.