In order to identify the role of H3 modification in regulating selected inflammation-related stemness genes, we measured the amount of ICAM3 DNA fragments in H3 modification marker pull-down DNAs in A549 lung cancer cells, MDA-MB-231 breast cancer cells and HepG2 liver cancer cells using the CHIP-qPCR assay. The gene discussed is ICAM3; the disease is breast cancer.