To investigate BIK autonomous effects on cellular growth properties in the absence of the pleiotropic effects of stressors, we generated doxycycline (Dox)-inducible BIK expression in the breast carcinoma cell lines MCF-7 and MDA-MB-231 (Fig. 1c and d), as representatives of ER-positive and triple-negative breast cancer (TNBC) subtypes, respectively. This evidence concerns the gene ESR1 and triple-negative breast carcinoma.