To determine whether miR-410 overexpression could promote EMT in vivo, SPC-A1-miR-410 cells were injected into the flanks of mice, and the A549-Inh subcutaneous tumor model established previously was also used.25 Consistent with the results in vitro, immunohistochemical staining of Vimentin and Slug was remarkably increased, whereas E-cadherin was significantly decreased in SPC-A1-miR-410 tumors (Fig. 6a). This evidence concerns the gene VIM and neoplasm.