A direct count of the number of cells which had invaded to the bottom surface of the membrane showed that there was no significant difference between Notch3IC and Control cells in invasion in Boyden chambers pre-coated with collagen I (p = 0.5634, Welch’s t-test), pre coated with Matrigel GFR, a laminin rich amalgam of ECM and tumor-derived components [47] (p = 0.8634), or uncoated Boyden chambers (p = 0.1205). Here, LAMB2 is linked to neoplasm.