RT–qPCR analysis revealed that the level of serum‐derived LINC00853 was similar in the two groups (Fig. 2A, P = 0.7246), whereas that of the serum EV‐derived LINC00853 (EV‐LINC00853) was significantly higher in patients with HCC than in healthy controls (Fig. 2E, P < 0.001). This evidence concerns the gene LINC00853 and hepatocellular carcinoma.