Minimal significant biological changes were seen between TAK1WT and TAK1KO cells with a 1.19 fold increase in angiopoietin-1 and decreases in expression of HB-EGF (1.23-fold), IGFBP-3 (1.09-fold), IL-8(1.05-fold) and thrombospondin (1.16-fold), suggesting that the TAK1 mediated angiogenetic signaling may occur outside of the cancer cells themselves (Figure 5C). The gene discussed is IGFBP3; the disease is cancer.