Another mutated gene, which was significantly associated with an immune-suppressed TIME in all HNSCC as well as in HPV-positive HNSCC, was FBXW7. Additional gene mutations with tendencies towards an immune-suppressed TIME in the HPV-positive subset were MAP3K13, MYO9A and NBPF1. EP300 was together with TP53 the only mutated gene, which was associated with one TIME subtype across all HNSCC, HPV-negative HNSCC and HPV–positive HNSCC. This evidence concerns the gene FBXW7 and head and neck squamous cell carcinoma.