Finally, to further evaluate the effect of AX09-induced antibody on the adhesion/spreading capacities of breast cancer cells, spheroids generated from MDA-MB-231 cells were assessed by a quantitative/dynamic monitoring cell system (xCELLigence Real-Time Cell Analysis, RTCA) in the presence of antibodies from mice immunized with AX09 or MS2 wt. The gene discussed is ADAM8; the disease is breast cancer.