RHOA and chronic obstructive pulmonary disease: Because we did not determine Akt and F-actin, the mechanism of AM and cytoskeletal rearrangement for PI3K/mTOR/RhoA pathway is unclear after the expressions of PI3K and mTOR were silenced, and their effect on interaction of mTORC1 and mTORC2 with RhoA is unclear in COPD.