We first established cell models with MTH1 or NUDT5 depletion using two ESCC cell lines (KYSE50 and KYSE70) transfection with either LV-MTH1-RNAi, LV-NUDT5-RNAi and control CON077-(hU6–MCS–Ubiquitin–EGFP–IRES–puromycin) and verified the changes in the expression by Western blotting. Here, NUDT1 is linked to esophageal squamous cell carcinoma.