The objectives of this study were I) to investigate the protein expression of EGR1 and EGR3 in gliomas, II) to compare protein expression levels in tumour cells located in central, intermediate and peripheral tumour areas to uncover potential differential expression patterns in migrating and non-migrating tumour cells, and III) to investigate the prognostic potential of EGR1 and EGR3, including their combined prognostic value. The gene discussed is EGR3; the disease is neoplasm.