MYO1B and infection: To generate host cells impacted by various combinations of parasite-dependent stimuli for downstream RNA-seq, we subjected the host cells to infection with either wild-type RH (type I) strain parasites, mutant Δmyr1 parasites lacking the MYR1 protein (constructed from an RH Δmyr1 mCherry parental strain [25]) (see Fig. S1 in the supplemental material), or a parasite-free cell lysate (i.e., mock infection) from feeder human foreskin fibroblast (HFF) cells used to maintain both parasite lines.