Using clonogenic assays, we first analysed the impact of targeting the major protein kinases involved in DNA DSB repair using specific and characterised inhibitors (ATMi, KU-55933; ATRi, VE-821; DNA-Pkcsi, KU-57788) on the survival of HPV-positive and HPV-negative HNSCC incubated with the inhibitors for 24 h in the absence of radiation, versus a vehicle-only control (DMSO). This evidence concerns the gene WEE1 and head and neck squamous cell carcinoma.