In addition, a recent study [48], investigating the involvement of RSU1 isoforms in cancer cell metastasis, utilized shRNA-mediated silencing to generate breast cancer cell lines that permanently lacked RSU1. RSU1 depletion in the two cell lines had completely opposite effects on cell migration, cell invasion and tumor spheroid invasion in 3D collagen gels. The gene discussed is RSU1; the disease is breast carcinoma.