Therefore, we used a plasmid to overexpress NRBP2-IRES2-eGFP (plasmid containing NRBP2 in the internal ribosome entry site (IRES; 1, 2) and the enhanced green fluorescent protein (eGFP) coding region) under control of the cytomegalovirus (CMV) promoter in D324 medulloblastoma cells. The gene discussed is NRBP2; the disease is medulloblastoma.