The methylation status of HAND2-AS1 promoter was further verified using an online software DiseaseMeth 2.0 [24], which showed that HAND2-AS1 is hypermethylated in several cancers, such as bladder carcinoma, lung adenocarcinoma, pancreatic adenocarcinoma, head and neck cancers, colon carcinoma, and anal carcinoma (Supplementary Figure S1b). This evidence concerns the gene HAND2 and anal carcinoma.