The dramatic band shift phenomena were also verified for endogenous ULK1 in human colorectal cancer cells (HCT 116) and human retinal pigmented epithelial cells (RPE1) (Fig 1F), as well as endogenous ULK1 and exogenous mULK1 in HEK-293T and HeLa cells (S1A and S1B Fig). This evidence concerns the gene ULK1 and colorectal cancer.