ERBB4 and hepatocellular carcinoma: Thus, in order to investigate clathrin dependency of the various receptor dimers, we used three HCC cell lines, Hep3B, HepG2 and PLC/PRF/5, that express varying levels of EGFR, ErbB2 and ErbB3, with no detectable ErbB4 expression, thus being representative of most HCC cell lines.