Thus, in order to investigate clathrin dependency of the various receptor dimers, we used three HCC cell lines, Hep3B, HepG2 and PLC/PRF/5, that express varying levels of EGFR, ErbB2 and ErbB3, with no detectable ErbB4 expression, thus being representative of most HCC cell lines. This evidence concerns the gene ERBB2 and hepatocellular carcinoma.