Additionally, we documented that mild and severe hypospadias express lower average transcript levels for AR and CYP19A1, whereas the expression of several steroidogenic enzymes, including CYP3A4, HSD17B14, HSD3B7, HSD17B7, and CYP11A1 are significantly decreased in severe hypospadias, compared with non‐hypospadiac tissues. Here, CYP3A4 is linked to hypospadias.