CFTR and cystic fibrosis: The CDC’s NSMBB Molecular Quality Improvement Program laboratory designed a study to evaluate the utility of nine DBS DNA extraction methods that range from a highly purified column extraction to a crude detergent-based no-wash boil prep and examines their utility in NGS using two assays to sequence the CFTR gene, MiSeqDx cystic fibrosis clinical sequencing assay (Illumina, San Diego, CA, USA) and Accel-AmpliconTM CFTR panel for Illumina platforms (Swift Biosciences, Ann Arbor, MI, USA).