Surprisingly, we didn’t observe any PD-L1 up-regulation of CARPD-L1z T cells either activated by CD3/CD28 antibodies or co-cultured with PD-L1+ tumor cells (Fig. 4e-f), even the percentage of CD25 and CD69 double-positive T cells confirmed they fully activated (Figure S8A-B). This evidence concerns the gene CD28 and neoplasm.