To investigate the role of PI3K in mediating the increase in TMEM16A and MUC5AC expression in response to EGF in HNECs, the HNECs from CRSwNP patients were pretreated for 30 min with PI3K inhibitor, LY294002 (25 μM), before treatment with EGF. This evidence concerns the gene ANO1 and chronic rhinosinusitis with nasal polyps.