As shown in Fig. 1a, incubation of HNECs derived from CRSwNP patients for 4 h with 50, 100 ng/ml EGF significantly increased the expression of TMEM16A mRNA; with 100 ng/ml EGF leading to a significant 2.2 ± 0.9-fold increase in TMEM16A mRNA, compared to control cells (p < 0.05). Here, ANO1 is linked to chronic rhinosinusitis with nasal polyps.