Three gene transcripts, MDM2 Binding Protein (MTBP), Pro-Melanin Concentrating Hormone (PMCH) and Fat mass and obesity-associated (FTO) were downregulated both in A549 parental and A549-∆ANXA1 cells, together with 626 other transcripts which were upregulated in both cell types, indicating that these transcripts are ANXA1-independent (Figure 1C). The gene discussed is ANXA1; the disease is obesity due to melanocortin 4 receptor deficiency.