Taken together, despite the fact that the cross-reactivity between ZIKV and secondary DENV infections still need to be well considered and evaluated, the ViroTrack Sero Zika IgG/IgM assay developed a rapid diagnostic assay for detecting ZIKV infection and potentially improved the distinguishability between the DENV and ZIKV infections. Here, CD40LG is linked to Zika virus infectious disease.