In serological study 3, four groups of samples—specifically, samples from primary ZIKV infections with no prior DENV infection (pZIKV); primary ZIKV infections with documented prior DENV infection (DENVpZIKV); primary DENV infections (pDENV); and secondary DENV infections (sDENV) in early convalescence were tested with ViroTrack Sero Zika IgG/IgM with the aim of assessing the cartridge specificity in relation to DENV infection. This evidence concerns the gene CD40LG and Zika virus infectious disease.