In our presented study, the ViroTrack Sero Zika IgM assay can clearly distinguish the ZIKV infection (group of both ZIKV primary infected and ZIKV-positive with previously DENV-infected samples) from the DENV infection (group of both primary and secondary DENV-only infected samples) with a sensitivity and a specificity of 96%, when sampling is collected at the convalescent-phase. This evidence concerns the gene CD40LG and Zika virus infectious disease.