Our results indicated that TQ and ω3 administration enhanced phosphorylation of AMPK, Sirt1, and PGC-1α expression in WAT, further elucidating the interplay of TQ and ω3 in promoting mitochondrial function and eventually contributing to a marked improvement in obesity and insulin resistance of HFD mice. The gene discussed is PPARGC1A; the disease is obesity due to melanocortin 4 receptor deficiency.