In the present study, we showed that a combination of TQ and ω3 administration in HFD mice enhanced the reduction of NOV/CCN3 and TWIST2 expression in both adipose and liver tissue, suggesting a potential capacity of the combination of TQ and ω3 in attenuating hypoxia-induced inflammatory responses, eventually contributing to the amelioration of obesity-associated insulin resistance. This evidence concerns the gene TWIST2 and obesity disorder.