To investigate the potential of PCa-specific SPOP mutations as radiosensitizing factors in PCa, we transfected DU145 and PC-3 cell lines with pMCV6 expression vectors encoding for WT or mutant (Y87N, K129E and F133V) SPOP and assessed the effect on cell response to 2–8 Gy γ irradiation via clonogenic assay. Here, SPOP is linked to posterior cortical atrophy.