As shown in Figure 5, the top ten significant pathways were as follows: systemic lupus erythematosus, spliceosomes, amoebiasis, African trypanosomiasis, porphyrin and chlorophyll metabolism, Amyotrophic lateral sclerosis (ALS), Malaria, Renin secretion, Taurine and hypotaurine metabolism, and Tuberculosis. The gene discussed is REN; the disease is malaria.