In this study, we enrolled 378 cases in The Cancer Genome Atlas (TCGA) database and a cohort including 73 well-documented, clinically annotated CRC tumor specimens to investigate the expression of PD-1 and Tim-3 and explore the relationship between PD-1 and Tim-3 and the prognostic value of PD-1 and Tim-3 expressions in stage I-III CRC. The gene discussed is HAVCR2; the disease is neoplasm.