FOXD4 and colorectal carcinoma: Moreover, the results of qPCR, immunohistochemistry staining and Western blot assay revealed that FOXD4, ENPEP, HOXC6, and ALOX15B are over expressed in CRC tissues and cells.The advantage of this study is that we identified a prognostic 4-gene signature that had relatively high AUCs for 1/3/5-year survival rate predictions in the training and validation data sets.