As PD-1 was increased on total CD4+ T cells, covering CD4+CXCR5+ and CD4+CXCR5− T cells, we analyzed ICOS expression also in these subpopulations separately in AChR-MG, SN-MG, and HC groups (Supplementary Figure 2). This evidence concerns the gene CXCR5 and myasthenia gravis.