(b) Next, by the targeted bisulfite sequencing and real‐time quantitative methylation‐specific polymerase chain reaction (RT‐qMSP), we further validated and confirmed that DLX5 hypermethylation was frequently occurred in AML and MDS, and observed that DLX5 methylation was associated with leukemia transformation in MDS. Here, DLX5 is linked to leukemia.