We also observed a lack of IL-10 release by established metastatic melanoma cell lines [21], whereas the incorporation of such a cell line (SK-MEL-28) into the Mel-RhS model resulted in marked up-regulation of IL-10 expression and release with melanoma cells, keratinocytes, and fibroblasts demonstrably expressing IL-10 mRNA in the Mel-RhS. This evidence concerns the gene IL10 and melanoma.