ZSCAN4 and head and neck squamous cell carcinoma: To study the human ZSCAN4 gene, we first sought to assess the expression of ZSCAN4 by screening a panel of HNSCC cell lines (012SCC, SCC13, Tu167, Tu159) using quantitative reverse transcription PCR (qRT-PCR; Fig. 1a) and immunoblot analysis (Fig. 1b).