Collectively, the results of the present study showed that F2R promoted malignancy and facilitated metastasis in glioma cells under the regulation of transcription factor SOX2 and triggering Wnt/β-catenin signaling pathway activation, suggesting F2R and its associated pathway is crucial for glioma tumorigenesis, and targeting this pathway may be pivotal in the treatment of glioma. This evidence concerns the gene F2R and central nervous system cancer.