Next, the analysis was extended and focused on the colon carcinoma cell lines Lovo (> 769,000 EpCAM/cell, ~ 182,000 EGFR/cell), LS174T (~ 602,000 EpCAM/cell, ~ 29,000 EGFR/cell) and HTC-116 (~ 591,000 EpCAM/cell, ~ 17,000 EGFR/cell) characterized by particular high EpCAM and high to moderate EGFR expression levels and the lung carcinoma cell lines A549 (~ 8,000 EpCAM/cell, ~ 80,000 EGFR/cell) and NCI-H460 (< 2,000 EpCAM/cell, ~ 42,000 EGFR/cell) presenting rather low EpCAM and moderate EGFR expression levels (Supplementary Table 1). The gene discussed is EGFR; the disease is colon carcinoma.