To further validate the role of IL-23 on DN T cells in lupus-prone mice, we overexpressed IL-23 in wild-type B6.lpr mice at 8 weeks of age by hydrodynamic administration of a minicircle (MC) cDNA construct encoding Il23p19 and Il12/23p40 (IL-23 MC)31 (Supplementary Fig. 7). This evidence concerns the gene IL23A and systemic lupus erythematosus.