During oocyst development and sporozoite formation, live cell imaging revealed NDC80–GFP fluorescence at multiple foci adjacent to the nuclear DNA at various stages of oocyst development from 7 days post-infection (d.p.i.)of the mosquito to 21 d.p.i., as well as a single focus in mature sporozoites (Fig. 5A,B). Here, NDC80 is linked to infection.