BIN1 and early-onset autosomal dominant Alzheimer disease: In total brain homogenates, we confirmed a trend towards reduction in BIN1 in severe relative to moderate stage Alzheimer’s disease and control tissues (Fig. 1A and B) and significant increases in total and phosphorylated tau amounts with increasing disease severity (Fig. 1A, C and D), as previously reported (Kurbatskaya et al., 2016).