Finally, the generation of 4N cells, either by silencing ESPL1/Separase or dihydrocytochalasin B (DCB) treatment, reveals that most newly-generated 4N cells rapidly reduce extra centrosomes over time, although a small fraction retains supernumerary centrosomes, thus resulting in CIN. The gene discussed is ESPL1; the disease is cervical squamous intraepithelial neoplasia.